| 中文名稱 | 半胱胺酸蛋白酶蛋白-12抗體 |
| 別 名 | UNQ9415; Apoptosis related cysteine protease; CASP 12; casp12; CASP12P1; Caspase 12 pseudogene 1; CASP-12; casp12; caspase 12 (gene/pseudogene); Caspase12; CASPC_HUMAN; OTTHUMP00000207026; OTTHUMP00000207028; OTTHUMP00000207031; OTTHUMP00000207032; OTTHUMP00000219058; UNQ9415. |
| 研究領(lǐng)域 | 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 46kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of human Caspase-12:201-300/420 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]. Function: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Tissue Specificity: Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: O08736 Gene ID: 100506742 Database links: Entrez Gene: 100506742 Human Entrez Gene: 12364 Mouse Entrez Gene: 156117 Rat SwissProt: Q6UXS9 Human SwissProt: O08736 Mouse SwissProt: Q920D5 Rat Omim: 608633 Human Unigene: 476989 Human Unigene: 81078 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Caspase-12是caspase家族成員，是存在于內(nèi)質(zhì)網(wǎng)上的促凋亡因子，內(nèi)質(zhì)網(wǎng)應(yīng)激早期可通過GRP（glucose-regulated protein，GRP）的表達(dá)增多來保護(hù)細(xì)胞；CASPASE-12是內(nèi)質(zhì)網(wǎng)細(xì)胞關(guān)鍵介導(dǎo)因子，caspase-12還是caspase-1的抑制劑。 |
| 產(chǎn)品圖片 |  Sample:Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-alpha smooth muscle Actin (bs-1105R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD  Sample:Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Caspase-12 (bs-1105R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45/52 kD Observed band size: 46 kD  Sample:Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Caspase-12 (bs-1105R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD  Paraformaldehyde-fixed, paraffin embedded (mouse fallopian tube); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (bs-1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (bs-1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caspase-12 Polyclonal Antibody, Unconjugated(bs-1105R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat testis tissue(high fat); 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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