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中文名稱

煙堿型乙酰膽堿受體α7抗體

別名

CHRFAM7A; ACHA7_HUMAN; cholinergic receptor, nicotinic, alpha 7; Neuronal acetylcholine receptor subunit alpha-7; ACHR ALPHA 7; AChR alpha 7 Receptor; Acra7; ALPHA-7NACHR; ALPHA7; ALPHA7 NICOTINIC ACETYLCHOLINE RECEPTOR; Alpha7 nicr; BTX; CHRNA7; CHRNA7-2; NACHR alpha7; NACHRA7; NARAD; Alpha 7 neuronal nicotinic acetylcholine receptor FAM7A hybrid; CHRNA7 (cholinergic receptor nicotinic alpha 7 exons 5 10) and FAM7A (family with sequence similarity 7A exons A E) fusion; CHRNA7; CHRNA7 DR1; CHRNA7 FAM7A fusion; CHRNA7 FAM7A fusion protein; D 10; D10; MGC120482; MGC120483.

研究領(lǐng)域	細(xì)胞生物神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡通道蛋白細(xì)胞膜受體
抗體來(lái)源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat, (predicted: Chicken, )
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	55kDa
細(xì)胞定位	細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human CHRNA7:441-502/502
亞型	IgG
純化方法	affinity purified by Protein A
儲(chǔ) 存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	The Nicotinic Acetylcholine Receptors are members of a superfamily of ligand gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be hetero pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C terminal extracellular region. The Nicotinic Acetylcholine Receptor alpha 7 forms a homo oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane. Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Subunit: Homopentamer. Interacts with RIC3; which is required for proper folding and assembly. Subcellular Location: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. SWISS: P36544 Gene ID: 1139 Database links: Entrez Gene: 1139 Human Entrez Gene: 374001 Chicken Entrez Gene: 282178 Cow Entrez Gene: 11441 Mouse Entrez Gene: 25302 Rat Omim: 118511 Human SwissProt: P22770 Chicken SwissProt: P54131 Cow SwissProt: P36544 Human SwissProt: Q8IUZ4 Human SwissProt: P49582 Mouse SwissProt: Q05941 Rat Unigene: 88 Cow Unigene: 511772 Human Unigene: 113464 Mouse Unigene: 9698 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片	Sample: Rrain(Mouse) lysate at 30ug; Primary: Anti- CHRNA7 (bs-1049R) at 1:300 dilution ; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 55 kD Observed band size: 55kDSample: Lane 1: Cerebrum (Rat) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Adrenal glands (Rat) Lysate at 40 ug Lane 4: Adrenal glands (Mouse) Lysate at 40 ug Lane 5: Placenta (Mouse) Lysate at 40 ug Lane 6: Lung (Mouse) Lysate at 40 ug Lane 7: Testis (Rat) Lysate at 40 ug Lane 8: HepG2 (Human) Cell Lysate at 30 ug Lane 9: SH-SY5Y (Human) Cell Lysate at 30 ug Primary: Anti-CHRNA7 (bs-1049R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56’50 kD Observed band size: 48 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- CHRNA7 (bs-1049R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 53 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Placenta (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Thymus (Mouse) Lysate at 40 ug Lymph node (Mouse) Lysate at 40 ug Primary: Anti- CHRNA7 (bs-1049R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 53 kD Sample: Lymph (Mouse) Lysate at 40 ug Primary: Anti- CHRNA7 (bs-1049R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(bs-1049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(bs-1049R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei