| 中文名稱 | 磷酸化干擾素調(diào)節(jié)因子7抗體 |
| 別 名 | Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF7A; IRF7; IRF7H. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 信號轉導 細胞凋亡 轉錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Pig, Cow, Horse, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 Flow-Cyt=1ug/test （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 54kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472:GV(p-S)(p-S)LD |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008] Function: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability. Subunit: Homodimer. Subcellular Location: Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus. Tissue Specificity: Expressed predominantly in spleen, thymus and peripheral blood leukocytes. Post-translational modifications: In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. TRAF6-mediated ubiquitination is required for IRF7 activation. Similarity: Belongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain. SWISS: Q92985 Gene ID: 3665 Database links: Entrez Gene: 3665 Human Entrez Gene: 54123 Mouse Entrez Gene: 293624 Rat Omim: 605047 Human SwissProt: Q92985 Human SwissProt: P70434 Mouse Unigene: 166120 Human Unigene: 3233 Mouse Unigene: 101159 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Uterus (Mouse) Lysate at 40 ug Large intestine (Mouse) Lysate at 40 ug Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 49kD  Sample:Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 48kD  Sample:Spleen (Mouse) Lysate at 40 ugPrimary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 49kD  Sample: Heart(Mouse) lysate at 30ug;Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (bs-3196R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 54kD Observed band size : 51kD  Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control:Molt-4.Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (bs-3196R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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