| 中文名稱(chēng) | 膜相關(guān)環(huán)指蛋白7抗體 |
| 別 名 | AXO; AXOT; Axotrophin; E3 ubiquitin-protein ligase MARCH7; MARCH VII; MARCH-VII; March7; MARH7_HUMAN; Membrane associated RING CH protein VII; Membrane associated ring finger (C3HC4) 7 E3 ubiquitin protein ligase; membrane associated ring finger C3HC4 7; Membrane associated RING finger protein 7; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; RING finger protein 177; RNF177. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 干細(xì)胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 78kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MARCH7/Axotrophin:31-130/704 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum. Function: E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Similarity: Contains 1 RING-CH-type zinc finger. SWISS: Q9H992 Gene ID: 64844 Database links: Entrez Gene: 64844 Human Entrez Gene: 57438 Mouse Entrez Gene: 311059 Rat Omim: 613334 Human SwissProt: Q9H992 Human SwissProt: Q9WV66 Mouse SwissProt: Q5R9W2 Orangutan SwissProt: Q5XI50 Rat Unigene: 529272 Human Unigene: 260635 Mouse Unigene: 117620 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Siha(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD  Sample:Siha(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD  Sample:Siha(human)cell Lysate at 30 ug MCF-7(human)cell Lysate at 30 ug U251(human)cell Lysate at 30 ug testis(rat) Lysate at 40 ug Primary: Anti-MARCH7 (bs-9341R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78kD Observed band size: 73 kD  Sample:Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78/74 kD Observed band size: 78/74 kD  Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. |
我要詢(xún)價(jià)
*聯(lián)系方式：
(可以是QQ、MSN、電子郵箱、電話(huà)等，您的聯(lián)系方式不會(huì)被公開(kāi))
*內(nèi)容：
