| 中文名稱 | P物質(zhì)受體抗體 |
| 別 名 | Neurokinin 1; Neurokinin 1 Receptor; NK 1 receptor; NK 1R; NK1 receptor; NK-1R; NK1R; NKIR; SPR; Substance P receptor; TAC 1R; TAC1R; Tachykinin 1 receptor; Tachykinin receptor 1; Tachykinin receptor 1; TACR1. |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 45kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPR:2-50/407 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. [provided by RefSeq, Sep 2008] Function: This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. Subunit: Interacts with ARRB1 Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25103 Gene ID: 6869 Database links: Entrez Gene: 6869 Human Entrez Gene: 21336 Mouse Entrez Gene: 24807 Rat Omim: 162323 Human SwissProt: P25103 Human SwissProt: P30548 Mouse SwissProt: P14600 Rat Unigene: 633301 Human Unigene: 8055 Mouse Unigene: 89609 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 此抗體與大、小鼠、及豚鼠均有交叉反應(yīng)。P物質(zhì)是由11個(gè)氨基酸組成的多肽，主要分布于感覺神經(jīng)纖維、后根神經(jīng)節(jié)和脊髓后角，作為重要的神經(jīng)遞質(zhì)或調(diào)制因子參與傷害性刺激的傳遞，該遞質(zhì)與突觸后膜上P物質(zhì)受體結(jié)合，將痛沖動(dòng)傳入中樞，產(chǎn)生疼痛反應(yīng)。 P物質(zhì)與神經(jīng)源性炎癥、瘙癢及疼痛的產(chǎn)生有關(guān)，通過刺激肥大細(xì)胞釋放組織胺、緩激肽及前列腺素等致炎、致敏、致痛物質(zhì)，引起一系列病理反應(yīng)，P物質(zhì)除作為傷害性信息的信使外，本身又為炎癥介質(zhì)。 |
| 產(chǎn)品圖片 |  Sample: U251 Cell Lysate at 40 ugPrimary: Anti-Neurokinin1 Receport (bs-0064R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 48 kD  Sample: Hela Cell Lysate at 40 ugPrimary: Anti-Neurokinin1 Receport (bs-0064R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SPR Polyclonal Antibody, Unconjugated(bs-0064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SPR Polyclonal Antibody, Unconjugated(bs-0064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SPR Polyclonal Antibody, Unconjugated(bs-0064R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei  Blank control: Hela.Primary Antibody (green line): Rabbit Anti-Neurokinin 1 Receptor antibody (bs-0064R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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