| 中文名稱(chēng) | 細(xì)胞外基質(zhì)蛋白1抗體 |
| 別 名 | Secretory Component Glycoprotein; ECM 1; Ecm1; ECM1_HUMAN; Extracellular matrix protein 1; Secretory component p85. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 細(xì)胞膜受體 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 59kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ECM1:488-567/567 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Extracellular matrix protein 1 (ECM1) This family consists of several eukaryotic extracellular matrix protein 1 (ECM1) sequences. ECM1 has been shown to regulate endochondral bone formation, stimulate the proliferation of endothelial cells and induce angiogenesis. Mutations in the ECM1 gene can cause lipoid proteinosis, a disorder which causes generalised thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Function: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Subunit: Interacts (via C-terminus) with HSPG2 (via C-terminus). Interacts with EFEMP1/FBLN3 and LAMB3. Interacts with MMP9. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. DISEASE: Lipoid proteinosis (LiP) [MIM:247100]: Rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. Note=The disease is caused by mutations affecting the gene represented in this entry. SWISS: Q61508 Gene ID: 1893 Database links: Entrez Gene: 1893 Human Entrez Gene: 116662 Rat GenBank: NP_073155 Human Omim: 602201 Human SwissProt: Q16610 Human SwissProt: Q62894 Rat Unigene: 81071 Human Unigene: 97792 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ECM1是一種分泌性糖蛋白，ECM1可促進(jìn)血管內(nèi)皮細(xì)胞的增殖和血管的生成, 經(jīng)研究發(fā)現(xiàn)，ECM1的表達(dá)可能與腫瘤及腫瘤的轉(zhuǎn)移有關(guān)聯(lián), 而目前在多種腫瘤的研究中也已確認(rèn)細(xì)胞外基質(zhì)與腫瘤的發(fā)生、發(fā)展及轉(zhuǎn)移等有密切的關(guān)系, ECM1是目前腫瘤研究的熱點(diǎn)。 |
| 產(chǎn)品圖片 |  Sample:MDA-MB-231(Mouse) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (bs-0776R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 65 kD  Sample:MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (bs-0776R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kD  Sample:HepG2(Human) Cell Lysate at 30 ug Primary: Anti-ECM1 (bs-0776R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kD  Sample:Skin(Mouse) Lysate at 40 ug Primary: Anti-ECM1 (bs-0776R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 64 kD  Tissue/cell: mouse endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ECM1 Polyclonal Antibody, Unconjugated(bs-0776R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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