| 中文名稱 | 白細(xì)胞介素10抗體 |
| 別 名 | Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Chicken, Pig, Rabbit, Sheep, Guinea Pig, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-10:101-178/178 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | IL-10 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Subunit: Homodimer. Subcellular Location: Secreted. Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. Similarity: Belongs to the IL-10 family. SWISS: P18893 Gene ID: 3586 Database links:
Entrez Gene: 3586 Human Entrez Gene: 16153 Mouse Entrez Gene: 25325 Rat Omim: 124092 Human SwissProt: P22301 Human SwissProt: P18893 Mouse SwissProt: P29456 Rat Unigene: 193717 Human Unigene: 874 Mouse Unigene: 9868 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 白細(xì)胞介素-10(IL-10)是主要由Th2細(xì)胞所產(chǎn)生的一種細(xì)胞因子，活化的B細(xì)胞、單核巨噬細(xì)胞和枯否氏細(xì)胞（Kupffer's cell）等也可產(chǎn)生IL-10。它是以抑制Th細(xì)胞克隆細(xì)胞因子合成為特點(diǎn)的多效免疫調(diào)節(jié)因子。 白介素-10是維護(hù)細(xì)胞因子網(wǎng)絡(luò)平衡的重要負(fù)調(diào)節(jié)因子。其作用機(jī)制可能是降低抗原遞呈細(xì)胞MHCⅡ類抗原表達(dá)，或誘導(dǎo)抗原遞呈細(xì)胞產(chǎn)生另一種細(xì)胞因子，改變細(xì)胞內(nèi)信號(hào)傳遞途徑，從而選擇性抑制某些細(xì)胞因子mRNA轉(zhuǎn)錄，并與Th2細(xì)胞產(chǎn)生的IL-4、IL-5有協(xié)同作用。 |
| 產(chǎn)品圖片 |  Sample:Lane 1: THP-1 (Human) Cell Lysate at 30 ug Lane 2: THP-1-LPS (Human) Cell Lysate at 30 ug Primary: Anti-IL-10 (bs-0698R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18.6 kD Observed band size: 17 kD  Sample:Spleen (Mouse) Lysate at 40 ug Primary: Anti-IL-10 (bs-0698R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD  Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(bs-0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(bs-0698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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