| 中文名稱 | 腸道內(nèi)富含的Kruppel樣因子/上皮鋅指蛋白4抗體 |
| 別 名 | Endothelial Kruppel like zinc finger protein; EZF; GKLF; KLF; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor); Kruppel like factor 4 (gut); Krueppel-like factor 4;EZF;GKLF;KLF 4;KLF-4;Krueppel like factor 4; KLF4_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 干細胞 細胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Chicken, Pig, Cow, Horse, Sheep, Guinea Pig, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 56kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLF4:415-513/513 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor. Function: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Subunit: Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Subcellular Location: Nucleus. Post-translational modifications: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: O43474 Gene ID: 9314 Database links: Entrez Gene: 9314 Human Entrez Gene: 16600 Mouse Omim: 602253 Human SwissProt: O43474 Human SwissProt: Q60793 Mouse Unigene: 376206 Human Unigene: 4325 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Klf4（原稱GKLF）調(diào)節(jié)體外細胞的增生與分化，有學者認為：KLF4可能參與消化系統(tǒng)腫瘤的發(fā)生及發(fā)展過程，多表達與結(jié)腸。又稱：上皮鋅指轉(zhuǎn)錄因子（上皮鋅指蛋白）Surface skin layer zinc finger protein。 |
| 產(chǎn)品圖片 |  Sample: 293T (human)Cell Lysate at 40 ugPrimary: Anti- KLF4 (bs-1064R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 60 kD  Sample:Hela Cell(Human)Lysate at 30 ug Primary: Anti- KLF4 (bs-1064R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56kD Observed band size: 56kD  Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF4) Polyclonal Antibody, Unconjugated (bs-1064R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KLF4 Polyclonal Antibody, Unconjugated(bs-1064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: MCF7(blue).Primary Antibody: Rabbit Anti-KLF4 AF647 Conjugated antibody (bs-1064R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-1064R-AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed. |
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