| 中文名稱 | DNA切除修復(fù)蛋白1抗體 |
| 別 名 | Excision Repair Cross Complementing 1; DNA excision repair protein ERCC-1; COFS4; UV20; DNA excision repair protein ERCC1; ERCC 1; Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1; Excision repair protein; UV 20; UV20;DNA excision repair protein ERCC-1. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ERCC1:151-250/297 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009] Subunit: Heterodimer composed of ERCC1 and XPF/ERRC4. Subcellular Location: Nucleus. Similarity: Belongs to the ERCC1/RAD10/SWI10 family. SWISS: P07992 Gene ID: 2067 Database links: Entrez Gene: 2067 Human Entrez Gene: 13870 Mouse Entrez Gene: 292673 Rat Omim: 126380 Human SwissProt: P07992 Human SwissProt: P07903 Mouse Unigene: 435981 Human Unigene: 280913 Mouse Unigene: 7320 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ERCC1蛋白是動(dòng)物內(nèi)重要的DNA修復(fù)基因家族成員之一。在人體內(nèi)它的低表達(dá)往往伴隨著基因不穩(wěn)定性增加,從而產(chǎn)生腫瘤惡性變的發(fā)生。ERCC1是損傷DNA核苷酸切除修復(fù)中高度保守的切除性核酶。在哺乳動(dòng)物的核苷酸切除修復(fù)中，XPG負(fù)責(zé)切割DNA損傷區(qū)域的3’端，而ERCC1-XPF復(fù)合物在5’端打開(kāi)切口。目前初步的研究表明，癌瘤中的ERCC1蛋白表達(dá)程度越低，順氯氨鉑化療的效果就越好。ERCC1蛋白陽(yáng)性的患者，非化療治療的預(yù)后要好于接受化療。 |
| 產(chǎn)品圖片 |  Sample:Hela(Human) Cell Lysate at 30 ug Primary: Anti-ERCC1 (bs-1726R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD  Sample:Testis (Mouse) Lysate at 40 ug Primary: Anti-ERCC1 (bs-1726R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD  Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERCC1) Polyclonal Antibody, Unconjugated (bs-1726R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERCC1) Polyclonal Antibody, Unconjugated (bs-1726R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ERCC1 Polyclonal Antibody, Unconjugated(bs-1726R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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