| 中文名稱 | 細胞角蛋白7抗體 |
| 別 名 | CK 7; CK-7; ck7; Cytokeratin 7; Cytokeratin-7; Cytokeratin7; D15Wsu77e; K2C7; K2C7_HUMAN; K7; Keratin 55k type ii cytoskeletal; Keratin 7; Keratin simple epithelial type 1 k7; Keratin type II cytoskeletal 7; Keratin type ii cytoskletal 7; Keratin, 55K type II cytoskeletal; Keratin, simple epithelial; Keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 7; Keratin-7; Keratin7; KRT 7; Krt2-7; KRT7; MGC11625; MGC129731; mgc3625; Sarcolectin; SCL; Type ii mesothelial keratin k7; Type-II keratin Kb7 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 54kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of mouse CK7:251-350/469 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Function: Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Subunit: Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. Post-translational modifications: Arg-20 is dimethylated, probably to asymmetric dimethylarginine. Similarity: Belongs to the intermediate filament family. SWISS: Q9DCV7 Gene ID: 110310 Database links: Entrez Gene: 3855 Human Entrez Gene: 110310 Mouse Entrez Gene: 300242 Rat Omim: 148059 Human SwissProt: P08729 Human SwissProt: Q9DCV7 Mouse SwissProt: Q6IG12 Rat Unigene: 411501 Human Unigene: 670221 Human Unigene: 289377 Mouse Unigene: 7913 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 結(jié)構(gòu)蛋白（Structural Proteins） CK-7是一種 54KDa 的中間絲蛋白，存在于大多數(shù)正常組織的腺上皮和移行上皮細胞中。 該抗體與多種良/惡性上皮性腫瘤反應(yīng)。腺癌中的卵巢、乳腺、肺的腺癌呈陽性反應(yīng)，而胃腸道的腺癌陰性。移行細胞腫瘤、前列腺癌也呈陽性反應(yīng)。通常認為 CK7是腺癌和移行上皮細胞癌的比較特異性的標(biāo)志。 |
| 產(chǎn)品圖片 |  Sample:Placenta (Mouse) Lysate at 30 ug Lung (Mouse) Lysate at 30 ug Primary: Anti-Cytokeratin 7 (bs-1744R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD  Sample: Large intestine (Mouse) Lysate at 30 ugPrimary: Anti- Cytokeratin 7 (bs-1744R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD  Sample:Large intestine (Mouse) Lysate at 40 ug Large intestine(Rat) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (bs-1744R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD  Sample:Embryo (Mouse) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (bs-1744R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cytokeratin 7 Polyclonal Antibody, Unconjugated(bs-1744R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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