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中文名稱

過氧化物酶體增殖物激活受體γ輔激活子1α抗體

別名

LEM6; PGC-1 Alpha; PGC1 Alpha; Ligand effect modulator 6; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1 ; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPARGC 1 alpha.

研究領(lǐng)域	免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子激酶和磷酸酶
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	88kDa
細(xì)胞定位	細(xì)胞核
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human PGC-1:601-700/798
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene. Function: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Subunit: Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with PRDM16. Interacts with LRPPRC. Homooligomer. Interacts with LPIN1. Subcellular Location: Nucleus. Tissue Specificity: Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. Post-translational modifications: hosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q9UBK2 Gene ID: 10891 Database links: Entrez Gene: 422815 Chicken Entrez Gene: 338446 Cow Entrez Gene: 479127 Dog Entrez Gene: 10891 Human Entrez Gene: 19017 Mouse Entrez Gene: 497232 Pig Entrez Gene: 83516 Rat Omim: 604517 Human SwissProt: Q865B7 Cow SwissProt: Q9UBK2 Human SwissProt: O70343 Mouse SwissProt: Q865B6 Pig SwissProt: Q9QYK2 Rat Unigene: 527078 Human Unigene: 259072 Mouse Unigene: 19172 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PGC-1α是一個新發(fā)現(xiàn)的轉(zhuǎn)錄激活因子,起到高游離脂肪酸刺激胰島α細(xì)胞分泌的重要調(diào)控作用。
產(chǎn)品圖片	Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-PPARGC1A (bs-1832R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-PPARGC1A (bs-1832R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 90 kD Sample: Heart (Mouse) Lysate at 30 ug Primary: Anti- PPARGC1A (bs-1832R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 88 kD Observed band size: 88 kD Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PPARGC1A/PGC1 alpha Polyclonal Antibody, Unconjugated(bs-1832R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PPARGC1A antibody (bs-1832R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.