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鋅指蛋白307抗體

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中文名稱 鋅指蛋白307抗體
別    名 zinc finger with KRAB and SCAN domains 4; Zinc finger protein 307; P373c6.1; ZKSCAN4; FLJ32136; P1P373C6; p373c6.1 (novel C2H2 type zinc finger protein); p373c6.1; Zinc finger protein 307; Zinc finger with KRAB and SCAN domains 4; ZKSCAN4; ZKSC4_HUMAN;dJ874C20.1; mszf35; SCAN-KRAB-zinc finger protein; Skz1; Zf47; Zfp-47; ZFP306; Zfp307; Zfp47; Zinc finger and SCAN domain containing protein 13; Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 306, isoform CRA_a; Zinc finger protein 307; Zinc finger protein 309; Zinc finger protein 47 homolog; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein zfp47; Zinc finger with KRAB and SCAN domains 3; ZKSC3_HUMAN; ZKSCAN3; ZNF309; ZSCAN13; ZSCAN35.  
研究領(lǐng)域 細胞生物  免疫學  染色質(zhì)和核信號  轉(zhuǎn)錄調(diào)節(jié)因子  鋅指蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse,  (predicted: Rat, Dog, Cow, Horse, Rabbit, )
產(chǎn)品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 62kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ZNF307:351-450/545 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 ZNF307 contains 1 SCAN box domain, 1 KRAB domain and 7 C2H2-type zinc fingers. It belongs to the Krueppel C2H2-type zinc-finger protein family and may be involved in transcriptional regulation. [SUBCELLULAR LOCATION] Nucleus. Expressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.

Function:
May be involved in the transcriptional activation of MDM2 and EP300 genes.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Contains 1 SCAN box domain.

SWISS:
Q969J2

Gene ID:
387032

Database links:

Entrez Gene: 387032 Human

Omim: 611643 Human

SwissProt: Q969J2 Human

Unigene: 44720 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產(chǎn)品圖片 Sample:
K562(Human) Cell Lysate at 40 ug
Primary: Anti-ZNF307/ZKSCAN3 (bs-1940R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse fetal liver; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated(bs-1940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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