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中文名稱

肝細(xì)胞核因子4α抗體

別名

FLJ39654; HNF-4-alpha; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4.

研究領(lǐng)域	腫瘤細(xì)胞生物免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子糖尿病
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat,
產(chǎn)品應(yīng)用	WB=1:500-1000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	52kDa
細(xì)胞定位	細(xì)胞核
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human HNF4 alpha:301-400/464
亞型	IgG
純化方法	affinity purified by Protein A
儲(chǔ) 存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene. Function: Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. Subunit: Homodimerization is required for HNF4-alpha to bind to its recognition site. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold. DISEASE: Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Similarity: Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P41235 Gene ID: 3172 Database links: Entrez Gene: 3172 Human Entrez Gene: 15378 Mouse Entrez Gene: 25735 Rat Omim: 600281 Human SwissProt: P41235 Human SwissProt: P49698 Mouse SwissProt: P22449 Rat Unigene: 116462 Human Unigene: 202383 Mouse Unigene: 12238 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 肝細(xì)胞核因子α(HNF4 α)是一類異質(zhì)的保守轉(zhuǎn)錄因子,參與調(diào)節(jié)肝臟內(nèi)糖代謝、脂代謝相關(guān)基因的表達(dá).HNF在胰島中也有表達(dá).HNF4-α目前用于2型糖尿病的研究也很多。
產(chǎn)品圖片	Sample: BRL-3A(Rat) Cell Lysate at 30 ug Primary: Anti-HNF4A (bs-3828R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Sample: Hcclm3 Cell (Human) Lysate at 40 ug Primary: Anti- HNF4A (bs-3828R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HNF4A) Polyclonal Antibody, Unconjugated (bs-3828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HNF4A Polyclonal Antibody, Unconjugated(bs-3828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Hep G2 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- EHNF4A antibody(bs-3828R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.