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含錨蛋白重復(fù)序列-細胞因子信號抑制物盒蛋白家族10抗體

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中文名稱 含錨蛋白重復(fù)序列-細胞因子信號抑制物盒蛋白家族10抗體
別    名 Ankyrin repeat and SOCS box containing 10; Ankyrin repeat and SOCS box protein 10; ASB 10; ASB-10; ASB10; ASB10_HUMAN.  
研究領(lǐng)域 細胞生物  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, )
產(chǎn)品應(yīng)用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 51kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ASB10.:531-450/467 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 ASB10 is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. SOCS boxes are carboxy terminal regions of homology found in the suppressor of cytokine signaling (SOCS) family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases. The SOCS box couples the suppressor of cytokine signalling proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation.

Function:
May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).

Similarity:
Contains 7 ANK repeats.
Contains 1 SOCS box domain.

SWISS:
Q8WXI3

Gene ID:
136371

Database links:

Entrez Gene: 136371 Human

Omim: 615054 Human

SwissProt: Q8WXI3 Human

Unigene: 647081 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產(chǎn)品圖片
Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (bs-4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (bs-4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- ASB10 (bs-4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- ASB10 (bs-4653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASB10) Polyclonal Antibody, Unconjugated (bs-4653R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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