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His Tag標(biāo)簽抗體

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中文名稱 His Tag標(biāo)簽抗體
別    名 HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag.  
產(chǎn)品類型 標(biāo)簽抗體 
研究領(lǐng)域  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Recombinant protein
產(chǎn)品應(yīng)用 WB=1:2000-5000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from His Tag: 
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

SWISS:
N/A

Gene ID:
N/A

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產(chǎn)品圖片 Sample:
His-tagged FMDV VP1 protein (type O) at 30 ug
Primary: Anti-His Tag  (bs-10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 47 kD
Sample:
His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4 ug
Primary: Anti-His Tag (bs-10582R) at 1/2000 ~ 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2 ug
Primary: Anti-His Tag (bs-10582R) at 1/2000 ~ 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-His tag (bs-10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
Primary: Anti-His tag (bs-10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD
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