| 中文名稱 | 脫氫酶/還原酶家族成員2抗體 |
| 別 名 | Dehydrogenase/reductase (SDR family) member 2; Dehydrogenase/reductase member 2; Dehydrogenase/reductase SDR family member 2; Dicarbonyl reductase HEP27; DHRSⅡ; Hep27; HEP27 protein; Protein D; SDR family member; SDR25C1; Short chain alcohol dehydrogenase family member; Short chain dehydrogenase/reductase family 25C member 1; DHRS2_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DHRS2:61-160/258 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | |
| 產(chǎn)品圖片 |  Protein: Hela(human) lysates at 40ug;Primary: rabbit Anti-DARC (bs-10771R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:27 kD Observed band size: 30 kD  Protein: lung(mouse) lysates at 40ug; Primary: Anti-DHRS2 (bs-10771R) at 1:300;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size :27 kD Observed band size :27 kD  Protein: liver(mouse) lysates at 30 ug;Primary: Anti-DHRS2 (bs-10771R) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 27 kD Observed band size : 27/75 kD  Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(bs-10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated(bs-10771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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