| 中文名稱 | 程序性死亡配體1抗體 |
| 別 名 | CD274; B7 H; B7 H1; B7 homolog 1; B7-H1; B7H; B7H1; CD 274; CD274 antigen; CD274 molecule; MGC142294; MGC142296; OTTHUMP00000021029; PD L1; PD1L1_HUMAN; PD1L1_Mouse; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL 1; PDL1; Programmed cell death 1 ligand 1; Programmed death ligand 1; RGD1566211. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Rat, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 32kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse B7-H1:201-290/290 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Programmed cell death 1 ligand 1(Programmed death ligand 1；PD-L1; PDCD1 ligand 1; B7 homolog 1; B7-H1; CD274) involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibit T-cell proliferation and cytokine production. Function: Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Subunit: Interacts with PDCD1. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Endomembrane system; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. Similarity: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9EP73 Gene ID: 60533 Database links: Entrez Gene: 29126 Human Entrez Gene: 60533 Mouse Entrez Gene: 499342 Rat Omim: 605402 Human SwissProt: Q9NZQ7 Human SwissProt: Q9EP73 Mouse Unigene: 521989 Human Unigene: 245363 Mouse Unigene: 228198 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 程序性死亡配體1（B7-H1）可以促進(jìn)上皮細(xì)胞的惡性轉(zhuǎn)化,保護(hù)表皮細(xì)胞免于失巢凋亡,在腫瘤的發(fā)生,進(jìn)展和轉(zhuǎn)歸中發(fā)揮重要作用. 目前對CD274信號通路的進(jìn)一步的研究必將為自身免疫性疾病、病毒感染性疾病和器官移植后排斥反應(yīng)中T細(xì)胞介導(dǎo)的免疫應(yīng)答調(diào)控提供了新的途徑.B7-H1在癌癥、類風(fēng)濕、HIV感染等疾病中發(fā)現(xiàn)B7-H1有負(fù)調(diào)節(jié)作用。對B7-H1路徑進(jìn)行調(diào)控有助于自身免疫病及惡性腫瘤的治療。 |
| 產(chǎn)品圖片 |  Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti- CD274 (bs-1103R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 32 kD Observed band size: 48 kD  Sample: Lymph node (Mouse) Lysate at 40 ugPrimary: Anti- CD274 (bs-1103R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 32 kD Observed band size: 48 kD  Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-CD274 Polyclonal Antibody, Unconjugated(bs-1103R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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