| 中文名稱 | G蛋白/鳥苷酸結(jié)合蛋白抗體 |
| 別 名 | G protein beta subunit GI/GS/GT; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide-binding protein G; GBB1_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein beta subunit GI:101-200/341 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | G protein beta subunit GI/GS/GT (guanine nucleotide-binding protein G). WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment. Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus). Post-translational modifications: Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP. Similarity: Belongs to the WD repeat G protein beta family. Contains 7 WD repeats. SWISS: P62879 Gene ID: 2782 Database links: Entrez Gene: 2782 Human Entrez Gene: 14688 Mouse Entrez Gene: 24400 Rat Omim: 139380 Human SwissProt: P62873 Human SwissProt: P62874 Mouse SwissProt: P54311 Rat Unigene: 430425 Human Unigene: 2344 Mouse Unigene: 126047 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. G蛋白又稱：鳥苷酸結(jié)合蛋白，存在于全身各個(gè)組織和細(xì)胞中，G蛋白在信息傳遞中起到很重要的作用,參與細(xì)胞內(nèi)外信息的相互傳導(dǎo)。 激素受體與腺苷酸環(huán)化酶是細(xì)胞膜上兩類分開(kāi)的蛋白質(zhì)。激素受體結(jié)合的部分在細(xì)胞膜的外表面，而腺苷環(huán)化酶在膜的胞漿面，在兩者之間存在一種起耦聯(lián)作用的調(diào)節(jié)蛋白——鳥苷酸結(jié)合蛋白（guanine nucleotide-binding regulatory protein）細(xì)胞受到各種刺激信息，包括化學(xué)性的激素和神經(jīng)遞質(zhì)及非化學(xué)性的聲音、圖像等。刺激信息通過(guò)G蛋白的激活作用，在胞漿中生成數(shù)目眾多的第二信使分子，產(chǎn)生十萬(wàn)級(jí)放大效應(yīng)，引起細(xì)胞興奮，讓后促動(dòng)器官完成各自功能。 1977年，美國(guó)科學(xué)家阿爾弗雷德-戈德曼-吉爾曼(Alfred Goodman Gilman)發(fā)現(xiàn)了G蛋白及其在細(xì)胞興奮傳導(dǎo)方面的重要作用。1994年，吉爾曼因“G蛋白”方面的研究成果而榮獲諾貝爾生理及醫(yī)學(xué)獎(jiǎng)。 |
| 產(chǎn)品圖片 |  Sample:Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GNB1 (bs-0348R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GNB1) Polyclonal Antibody, Unconjugated (bs-0348R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-G protein beta subunit GI Polyclonal Antibody, Unconjugated(bs-0348R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-G protein beta subunit GI Polyclonal Antibody, Unconjugated(bs-0348R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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