| 中文名稱 | 谷氨酸半胱氨酸γ合成酶抗體 |
| 別 名 | CEGT_HUMAN; UGCG; GCS; GLCT 1; GLCT-1; GLCT1; Glucosylceramide synthase; UDP glucose ceramide glucosyltransferase; UDP glucose N acylsphingosine D glucosyltransferase; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase; Ugcg. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Cow, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCS:21-120/394 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | GCS (glucosylceramide synthase ) may serve as a 'flippase' as well as a glucosyltransferase that transfers glucose to ceramide. Able to use UDP-galactose to synthesize galactosylceramide with 10% of efficiency with which it utilizes UDP-glucose. [Catalytic activity] UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Lipid metabolism; sphingolipid metabolism. [Subcellular location] Endoplasmic reticulum membrane; Multi-pass membrane protein. Belongs to the glycosyltransferase 2 family. Function: Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Found in all tissues examined. Similarity: Belongs to the glycosyltransferase 2 family. SWISS: Q16739 Gene ID: 7357 Database links: Entrez Gene: 7357 Human Entrez Gene: 22234 Mouse Entrez Gene: 83626 Rat Omim: 602874 Human SwissProt: Q16739 Human SwissProt: O88693 Mouse SwissProt: Q9R0E0 Rat Unigene: 304249 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 谷氨酸半胱氨酸γ合成酶又稱葡萄糖神經(jīng)酰胺合成酶(glucosylceramide synthase, GCS)是調(diào)控神經(jīng)酰胺代謝的關(guān)鍵酶之一, 其活性水平與腫瘤細(xì)胞多藥耐藥有密切關(guān)系. 有研究報道GCS在人卵巢癌細(xì)胞、口腔表皮癌細(xì)胞 、黑色素瘤細(xì)胞、乳腺癌細(xì)胞及膽囊癌、胃癌中均有表達(dá). |
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-0701R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (bs-0701R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GCS/glucosylceramide synthase Polyclonal Antibody, Unconjugated(bs-0701R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: mouse splenocytes(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). |
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