| 中文名稱 | G蛋白偶合受體激酶1抗體 |
| 別 名 | G-protein coupled receptor kinase 1; GRK1; GPRK1; RK; Grk1; Rhok; RHODOPSIN KINASE; RK_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 細(xì)胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Chicken, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 62kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRK1:201-300/563 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq] Function: Phosphorylates rhodopsin thereby initiating its deactivation. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. Subcellular Location: Membrane. Tissue Specificity: Retina and pineal gland. Post-translational modifications: Autophosphorylated. Farnesylation is required for full activity. DISEASE: Defects in GRK1 are a cause of congenital stationary night blindness Oguchi type 2 (CSNBO2) [MIM:613411]. It is non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia. Congenital stationary night blindness Oguchi type is associated with fundus discoloration and abnormally slow dark adaptation. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain. SWISS: Q15835 Gene ID: 6011 Database links: Entrez Gene: 6011 Human Entrez Gene: 24013 Mouse Omim: 180381 Human SwissProt: Q15835 Human SwissProt: Q9WVL4 Mouse Unigene: 103501 Human Unigene: 721727 Human Unigene: 257501 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. G蛋白偶聯(lián)受體激酶1(GRK1)是催化激動劑誘導(dǎo)的GPCR磷酸化以及啟動GPCR脫敏的關(guān)鍵激酶,主要用于GRK細(xì)胞膜轉(zhuǎn)位和對GRK功能的影響. |
| 產(chǎn)品圖片 |  Sample: Raji Cell Lysate at 40 ugPrimary: Anti-GRK1 (bs-1082R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 62 kD Observed band size: 62 kD  Sample:K562 Cell (Human) Lysate at 30 ug Primary: Anti-GRK1 (bs-1082R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 65 kD  Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated(bs-1082R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated(bs-1082R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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