| 中文名稱 | 胰島素樣生長因子結(jié)合蛋白5抗體 |
| 別 名 | IBP 5; IBP5; IGF binding protein 5; IGF BP5; Insulin Like Growth Factor Binding Protein 5; IBP5_HUMAN; Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 30kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IGFBP5:101-200/272 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Subcellular Location: Secreted. Tissue Specificity: Osteosarcoma, and at lower levels in liver, kidney and brain. Similarity: Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain. SWISS: P24593 Gene ID: 3488 Database links: Entrez Gene: 3488 Human Entrez Gene: 16011 Mouse Entrez Gene: 25285 Rat Omim: 146734 Human SwissProt: P24593 Human SwissProt: Q07079 Mouse SwissProt: P24594 Rat Unigene: 607212 Human Unigene: 405761 Mouse Unigene: 1593 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 生長因子和激素（ Growth Factor and Hormones） IGFBP5胰島素樣生長因子結(jié)合蛋白5(IGFBP-5)是一種可以與胰島素樣生長因子(IGFs)結(jié)合的分泌性蛋白,其通過IGFs調(diào)節(jié)細(xì)胞的生長、分化、凋亡、黏附和運(yùn)動,IGFBP-5與IGFs結(jié)合可以調(diào)節(jié)IGFs與IGF-I型受體間的相互作用,改變其促有絲分裂效應(yīng)、凋亡抑制效應(yīng)和促細(xì)胞運(yùn)動作用。 有人認(rèn)為：由于造骨細(xì)胞能表達(dá)與IGF-1受體截然不同的IGFBP-5受體，IGFBP-5本身也是一種生長因子，能夠不依賴IGF-I獨(dú)立作用來調(diào)節(jié)骨代謝。 |
| 產(chǎn)品圖片 |  Sample:Kidney (Mouse) Lysate at 40 ug Primary: Anti- IGFBP5 (bs-0406R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD  Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated(bs-0406R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated(bs-0406R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human nasopharyngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP5/IBP5 Polyclonal Antibody, Unconjugated(bs-0406R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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