| 中文名稱 | 鈉鉀ATP酶通道蛋白（內參）抗體 |
| 別 名 | adhesion molecule on glia; Na+K+ATPase; AMOG; AT1B2; AT1B2_HUMAN; ATP1B2; ATPase Na+/K+ transporting beta 2 polypeptide; ATPB2; ATPB2S; MGC93648; Na+/K+ -ATPase beta 2 subunit; Na, K ATPase beta 2 polypeptide; RATATPB2S; sodium potassium ATPase subunit beta 2 (non-catalytic); sodium pump subunit beta 2; sodium/potassium dependent ATPase beta 2 subunit; Sodium/potassium dependent ATPase subunit beta 2; sodium/potassium transporting ATPase beta 2 chain; sodium/potassium transporting ATPase subunit beta 2; Sodium/potassium-dependent ATPase subunit beta-2; Sodium/potassium-transporting ATPase subunit beta-2. |
| 產品類型 | 內參抗體 |
| 研究領域 | 通道蛋白 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 33kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATP1b2:201-290/290 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. [provided by RefSeq, Jul 2008] Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Subunit: Composed of three subunits: alpha (catalytic), beta and gamma. Subcellular Location: Membrane; Single-pass type II membrane protein. Similarity: Belongs to the X(+)/potassium ATPases subunit beta family. SWISS: P14415 Gene ID: 482 Database links: Entrez Gene: 482 Human Omim: 182331 Human SwissProt: P14415 Human Unigene: 643540 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 通道蛋白（Channel Protein） 鈉鉀ATP酶是位于細胞膜上的一種糖蛋白,與ATP 的分解和細胞內外鈉、鉀離子的轉運密切相關,哺乳動物各種組織細胞的鈉鉀ATP 酶的免疫學特性基本相同。 |
| 產品圖片 |  Sample:A549(Human) Cell Lysate at 30 ug Primary: Anti-ATPase Na+/ K+ beta 2 (bs-1152R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample: NIH/3T3(mouse) Cell Lysate at 40 ugPrimary: Anti-ATPaseNa＋/K＋ beta2 (bs-1152R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated(bs-1152R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated(bs-1152R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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