| 中文名稱 | 三葉肽因子3抗體 |
| 別 名 | Trefoil Factor 3; Trefoil factor3; hP1.B; Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; Polypeptide P1.B; TFF3; TFI; TREFOIL; Trefoil factor (intestinal). |
| 研究領(lǐng)域 | 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 6.5kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TFF3:221-320/923 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] Subunit: Monomer. Homodimer; disulfide-linked. Subcellular Location: Secreted, extracellular space, extracellular matrix, apical lamina. Tissue Specificity: Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Similarity: Contains 1 P-type (trefoil) domain. SWISS: Q07654 Gene ID: 7033 Database links: Entrez Gene: 7033 Human Omim: 600633 Human SwissProt: Q07654 Human Unigene: 82961 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 三葉草肽Ⅲ是三葉肽中結(jié)構(gòu)獨(dú)特的一類小分子肽,它對(duì)于胃黏膜保護(hù)及損傷后修復(fù)具有重要作用,它的作用機(jī)制復(fù)雜,通過(guò)與其受體作用機(jī)制及信號(hào)傳導(dǎo)機(jī)制的研究,以加深其對(duì)胃黏膜保護(hù)機(jī)制的認(rèn)識(shí)。在促進(jìn)黏膜愈合的治療上,三葉肽是一個(gè)新的思路。 局部應(yīng)用三葉肽可能使黏膜凝膠產(chǎn)生更大的穩(wěn)定性,使胃黏膜對(duì)各種損傷因子的防御能力增強(qiáng)。三葉肽在誘導(dǎo)上皮修復(fù)上的直接作用,可用于許多胃腸道疾病。它作為輔助劑或取代皮質(zhì)類固醇或其他免疫抑制劑，在潰瘍病及炎癥性腸病的治療上可能是一個(gè)突破。三葉肽研究可能對(duì)胃十二指腸潰瘍和炎癥、壞死性小腸結(jié)腸炎、放療和化療導(dǎo)致的黏膜炎帶來(lái)新的治療方法。 三葉草肽Ⅲ與黏液糖蛋白相互作用,可誘導(dǎo)上皮細(xì)胞遷移，參與信號(hào)傳導(dǎo)機(jī)制，調(diào)節(jié)胃酸及運(yùn)動(dòng)功能。 |
| 產(chǎn)品圖片 |  Sample:Small intestine (Mouse) Lysate at 40 ug Small intestine (Rat) Lysate at 40 ug Primary: Anti-TFF3 (bs-0535R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 6.5 kD Observed band size: 16 kD  Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(bs-0535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: mouse small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(bs-0535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.  Cell:HT-29Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of primary antibody (Cat#: bs-0535R) on HT-29(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody . |
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