| Synonyms: | KHOS/NP（人骨肉瘤細胞） |
| Background: | This cell line was derived from HOS (ATCC CRL-1543) by transformation using Kirsten murine sarcoma virus (Ki-MSV). |
| Species: | Homo sapiens, human |
| Tissue: | bone |
| Disease: | osteosarcoma |
| Gender: | female， 13 years， Caucasian |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18 Cell Line Authentication Service |
| Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) We strongly suggest to purchase the complete medium |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Tumor Formation: | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments: | The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice. Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens. |
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