| I. General information | |
| Synonyms: | TSA201 |
| Background: | tsA201 is a transformed human kidney (293, ECACC catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling. |
| Species: | human (Homo sapiens) |
| Tissue: | Kidney |
| Disease: | Human embryonal kidney, SV40 transformed |
| Gender: | N/A |
| Morphology: | Epithelial-like |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 9,13 D5S818: 8,9 D7S820: 11 THO1: 7,9.3 TPOX: 11 vWA: 16,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: | DMEM+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:8;i.e. seeding at 2-4x10,000 cells/cm² using ; mechanical agitation or EDTA solution; 5% CO2; 37°C. Do not let cells become over confluent as this can affect morphology. |
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