Synonyms: | SV40LT-SMC Clone HEP-SA (大鼠平滑肌細(xì)胞) |
Background: | This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. |
Species: | Rattus norvegicus, rat |
Tissue: | aorta; smooth muscle |
Gender: | 3 months, rat |
Morphology: | fibroblast |
Growth Mode: | adherent |
DNA Profile: | Cell Line Authentication Service |
Culture Medium: | DMEM+10%NBCS+200 mcg/mL G418 We strongly suggest to purchase the complete medium |
Cryopreservation medium: | 90%FBS+10%DMSO |
Genes Expressed: | alpha actin; large T antigen |
Cellular Products: | alpha actin; large T antigen |
Comments: | This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance). Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin. Large T antigen is detectable in the nuclei. |
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