| Synonyms: | SVCT人SV40轉(zhuǎn)化乳腺上皮細胞 |
| Background: | SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent. |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | Human breast epithelium, SV40 transformed |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS Cell Line Authentication Service |
| Culture Medium: | DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS We strongly suggest to purchase the complete medium |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
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