D-Luciferin sodium salt
| 別名: | D-蟲熒光素鈉鹽 | ||
|---|---|---|---|
| Cas號(hào): | 103404-75-7 | ||
| 分子式: | C11H7N2NaO3S2 | ||
| 分子量: | 302.3 | ||
| MDL號(hào): | MFCD00044938 | 儲(chǔ)存條件: | -20°C |
說(shuō)明：D-Luciferin sodium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects[1].
實(shí)驗(yàn)參考方法：
Mice[3]
In vivo BLI is performed using a cooled charge-coupled device camera system (IVIS Imaging System 100) 3, 5, 7, 10, 12, 14, 19, 21, 24, and 28 days after the inoculation of HCT116-Luc cells. Mice are injected with 75 mg/kg D-luciferin in 100 μL of phosphate-buffered saline subcutaneously near the scapula and were placed in the light-tight chamber of the imaging system. Beginning 5 min after injection, dorsal luminescent images with an exposure time of 1 s are acquired sequentially at a rate of one image per min until 20 min after D-luciferin injection. Data acquisition is continued until 40 min postinjection on days 3 or 5 and until 25 min on day 7, because of the prolonged time course of light emission. Binning is 4 and the field of view is 15 cm.
本實(shí)驗(yàn)參考方法僅供參考，雅吉生物未作認(rèn)證。
