| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Sheep, Goat, (predicted: Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Beta-lactoglobulin: |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | bov beta-lactoglobulin is the content of the milk in the category of a protein myosin. Protein contained in whole milk (3.7 percent) to 80% of the casein in the removal of the casein whey protein, 55% of the beta-lactoglobulin. Access to the crystallization of the isoelectric point = 5.2, the pH in this dual capacity under the main structure. The people in latex-free Beta-lactoglobulin. The beta-lactoglobulin and bov beta-lactoglobulinA-based. Function: Primary component of whey, it binds retinol and is probably involved in the transport of that molecule. Subcellular Location: Secreted. Tissue Specificity: Synthesized in mammary gland and secreted in milk. Post-translational modifications: Alternate disulfide bonds occur in equal amounts in all variants examined. Similarity: Belongs to the calycin superfamily. Lipocalin family. SWISS: P02754 Gene ID: 280838 Database links: Entrez Gene: 280838 Cow SwissProt: Q32P89 Cow Unigene: 385 Cow
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產(chǎn)品圖片 |  Sample: Goat milk at 30ug; Primary: Anti- Beta-lactoglobulin (bs-0814R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 18kD Observed band size : 18kD  Paraformaldehyde-fixed, paraffin embedded (sheep breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta-lactoglobulin) Polyclonal Antibody, Unconjugated (bs-0814R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (sheep breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta-lactoglobulin) Polyclonal Antibody, Unconjugated (bs-0814R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. |
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