Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Embryonic chicken retinal cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.^1,2

Application References

(PubMed link indicates BioLegend citation)

1. Eisenbarth GS, et al. 1979. Proc. Natl. Acad. Sci. 76:4913. (IF)
2. Eisenbarth GS, et al. 1982. Proc. Natl. Acad. Sci. 79:5066. (IF)
3. Russell TR, et al. 1984. Cytometry 5:539. (FC)

RRID

AB_2566394 (BioLegend Cat. No. 150703) AB_2566395 (BioLegend Cat. No. 150704)