Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full length human α-tubulin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
ICC - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 ?g per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2?- 10??g/mL is suggested. For immunofluorescent staining applications, a concentration range of 1 - 4 ?g/ml is recommended.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
- Product Citations
-
- RRID
- AB_439760 (BioLegend Cat. No. 627901) AB_439761 (BioLegend Cat. No. 627902)
Antigen Details
- Structure
- Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
- Distribution
-
Ubiquitous
- Function
- Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
- Interaction
- RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
- Modification
- Phosphorylation, acetylation
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96. - Gene ID
- 84790 View all products for this Gene ID
- UniProt
- View information about Tubulin-alpha on UniProt.org
