Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

CKB mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, blocking of MHC class II - restricted CD4⁺ T cell responses^2,3, induction of B cell differentiation⁴, and immunohistochemical staining⁵ of acetone-fixed frozen sections.

This clone does not cross react with other haplotypes (e.g., b, d, f, p, q, s).

Application References

(PubMed link indicates BioLegend citation)

1. Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP)
2. Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438.
3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
4. Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933.
5. Ardehali A, et al. 1995. Circulation 92:450. (IHC)
6. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
7. Zhang L, et al. 2011. Lupus. 20:667. PubMed.

Product Citations

1. Zhang L, et al. 2011. Lupus. 1.296527778. PubMed
2. Hausmann A, et al. 2021. J Exp Med. 218:. PubMed
3. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed

RRID

AB_313459 (BioLegend Cat. No. 110002)