Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human Adiponectin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.05-0.2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of Adiponectin protein ranging from 10,000 to 156 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated 112-1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified 62-9 antibody as the capture antibody and human Adiponectin protein as the standard.

For testing human Adiponectin (Acrp30) in serum, plasma, or supernatant, BioLegend’s ELISA LEGEND MAX? Kits are specially developed and recommended.

RRID

AB_2571888 (BioLegend Cat. No. 528803) AB_2571889 (BioLegend Cat. No. 528804)