Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Aqueous buffered solution containing a carrier protein and preservatives.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

Store between 2-8°C. Do not store antibody diluted below 50 ug/mL in the absence of protein (i.e.: add 2% bovine serum albumin).

Application

IHC-P - Quality tested
Dot Blot, ELISA, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.

Application References

(PubMed link indicates BioLegend citation)

1. Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed
2. Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Blot) PubMed
3. Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed
4. Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP)
5. Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed
6. Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed

Product Citations

1. Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed
2. Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed
3. Cheng-Hathaway PJ, et al. 2018. Mol Neurodegener. 13:e0200060. PubMed

RRID

AB_2564979 (BioLegend Cat. No. 805407)