Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution (no preservatives or carrier proteins).

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

Do not store antibody diluted below 50 ?g/mL in the absence of protein (i.e.: add 2% bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

Application

IHC-P -　Quality tested
DOT Blot, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.

Application References

(PubMed link indicates BioLegend citation)

1. Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed
2. Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Plot) PubMed
3. Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed
4. Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP)
5. Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed
6. Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed

Product Citations

1. Vingtdeux V, et al. 2011. FASEB J. 25:219-231. PubMed
2. Watts J, et al. 2014. Proc Natl Acad Sci U S A. 111:10323-10328. PubMed
3. Lisa Dolfe et al. 2018. Journal of Alzheimer's disease reports. 2(1):27-39 . PubMed
4. Diomede L, et al. 2014. Neurobiol Dis. 62:521-532. PubMed
5. Watanabe H, et al. 2012. J Neurosci. 32:5085-5096. PubMed
6. Voth S, et al. 2020. FASEB J. 9156:34. PubMed
7. Shi H, et al. 2020. Acta Neuropathol. 139:813. PubMed
8. Bourassa P, et al. 2020. Neuropathol Appl Neurobiol. 46:458. PubMed
9. Plowey ED, et al. 2022. Acta Neuropathol. 144:143. PubMed
10. Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed
11. Kim K, et al. 2020. Nat Commun. 0.540972222. PubMed
12. Shi H, et al. 2020. Acta Neuropathol Commun. 0.473611111. PubMed
13. Ohmi K, et al. 2011. PLoS One. 6:e27461. PubMed
14. Parker H, et al. 2020. EMBO Mol Med. 12:e11185. PubMed
15. Ruiz–Riquelme A, et al. 2018. Acta Neuropathol Commun. 6:26. PubMed
16. Carreras I, et al. 2019. Sci Rep. 9:10972. PubMed

RRID

AB_2734555 (BioLegend Cat. No. 805409) AB_2564680 (BioLegend Cat. No. 805401) AB_2564681 (BioLegend Cat. No. 805402) AB_2564679 (BioLegend Cat. No. 805403)